Coding

Part:BBa_K3646000:Design

Designed by: Sukanya Chakraborty   Group: iGEM20_IISER_Berhampur   (2020-10-25)


DENV-NS5 CDS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 79
    Illegal EcoRI site found at 1509
    Illegal XbaI site found at 1162
    Illegal XbaI site found at 1455
    Illegal XbaI site found at 2643
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 79
    Illegal EcoRI site found at 1509
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 79
    Illegal EcoRI site found at 1509
    Illegal BamHI site found at 2548
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 79
    Illegal EcoRI site found at 1509
    Illegal XbaI site found at 1162
    Illegal XbaI site found at 1455
    Illegal XbaI site found at 2643
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 79
    Illegal EcoRI site found at 1509
    Illegal XbaI site found at 1162
    Illegal XbaI site found at 1455
    Illegal XbaI site found at 2643
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1749
    Illegal BsaI.rc site found at 1909
    Illegal BsaI.rc site found at 2315


Design Notes

Gene synthesized using Splicing by Overlap Extension. Used as a part of FRaPPe combined CID-FRET reporter to study host-viral PPIs in mammalian HEK293 cells.

Source

Gene sequence of Dengue Virus Non-Structural Protein 5 was sourced from https://www.ncbi.nlm.nih.gov. TSV08 Strain, Serotype 1, GenBank Accession : KR919821.1

References